FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27595396
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC118500.0124.305465
CACGACG120000.0122.5697564
CGACGCT123000.0118.9325946
GACGCTC138200.0106.0615547
CTACACG142150.0104.565541
ACACGAC141350.0104.312623
TACACGA151800.097.704732
ACGCTCT154850.094.42398
CGCTCTT177400.082.216849
TCCGATC148450.019.07346715-19
TTCCGAT157650.018.7698810-14
CTTCCGA163900.018.2398910-14
TCTTCCG177400.016.81915910-14
CCGATCT177450.016.3893815-19
CGCGCAT12550.015.752861
CGCCGTA333550.014.8354460-64
CGCGTAC8451.0913936E-1114.7309791
TCGCCGT339250.014.50497860-64
GCCGTAT341300.014.43059860-64
CGTATCA346950.014.09993665-69
Produced by FastQC (version 0.11.5)