FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005403572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169089394
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA543600.0126.021441
CGATTCG415000.0124.804654
CGATCGA443500.0120.2757645
GACGCGT373500.0120.2039641
CGTATCG240600.0118.274834
GACACGC1066400.0117.5305251
CGCGATT479750.0116.152022
CGGACGT390300.0115.6512151
GACGGCT930400.0114.974161
CGTCGAC245450.0114.9082267
TCCGCGA287800.0114.093368
ACGATAC587750.0112.904531
CCGATCG267950.0112.860324
TCGTTCG317350.0112.377558
ACCGTAA826600.0109.650831
CAGCGAC1054550.0107.549311
TACTCGC638700.0106.992721
GGCCGAT649550.0106.811891
TAAACCG537500.0106.016021
GCGACGT470950.0105.751683