FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005403573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169089394
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC492100.0116.242745
CGACGCT505850.0112.18026
CACGACG513300.0111.8224644
GACGCTC616900.092.127337
ACACGAC635300.090.519643
CTACACG643550.090.1825561
TACACGA659700.087.688162
ACGCTCT705450.080.563538
CGCTCTT841050.067.6776669
TTCGCGA63650.027.7891962
CGCGAAA85150.027.6039451
TCGCGAC70450.024.7982043
TTTCGCG77250.022.8438511
CGCCGTA4112900.020.55030660-64
GCCCGAT205150.020.3886662
TCGCCGT4140950.020.32917460-64
GCCGTAT4165100.020.18832660-64
CGTATCA4188750.020.02565665-69
GTCGCCG4230400.019.96306860-64
TGGTCGC4356250.019.42652955-59
Produced by FastQC (version 0.11.5)