FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005403575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70588546
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC265450.0119.176255
CACGACG268550.0117.853614
CGACGCT270100.0116.641436
ACACGAC319400.099.680783
CTACACG324800.098.931811
GACGCTC322650.098.070927
TACACGA334350.095.7008742
ACGCTCT354600.088.989788
CGCTCTT411500.076.613959
ACGCGAC34300.019.2323442
CGCGACT37250.018.4876353
TCCGATC339950.018.08682415-19
CGCCGTA1130800.017.85790460-64
TCGCCGT1145400.017.544260-64
TTCCGAT367000.017.41756410-14
GCCGTAT1152950.017.41422360-64
CGTATCA1163300.017.20236665-69
CTTCCGA376500.017.12440910-14
GTCGCCG1179600.017.10806360-64
CCGTATC1158050.016.6155365-69
Produced by FastQC (version 0.11.5)