FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27613439
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA84350.0131.166232
CGTACCG57200.0130.39767
CGGCTAG220450.0126.6001361
TAGTCGA168050.0125.690165
CCGATCG56600.0125.183044
TCGCGAG125550.0125.018381
CTTACCG159700.0124.604311
TAGGTCG125800.0124.0548259
TATCGCG58650.0123.836866
TAACGCG50850.0123.270772
CGTACGT69950.0121.551567
TCGTCGC48550.0121.530546
ACGTACG70950.0119.531866
CTAGTCG86500.0117.9039155
CGCAACT129100.0117.507372
GTACCGT132200.0117.4468
GATCGAT144000.0117.012121
AATCGCG51850.0117.011079
ATCGTTA181050.0116.7452557
TCTACGG144950.0116.166598
Produced by FastQC (version 0.11.5)