FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27613439
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC142450.0124.678735
CGACGCT142300.0124.453566
CACGACG143600.0123.982714
GACGCTC162450.0109.507447
ACACGAC169100.0105.929163
CTACACG172350.0104.56361
TACACGA176600.0101.594842
ACGCTCT182800.097.03948
CGCTCTT203850.086.804759
TCCGATC177000.019.4044615-19
TTCCGAT183550.019.26504510-14
CTTCCGA188450.018.94876310-14
CGGTACG9350.018.6074494
TCTTCCG203150.017.69181310-14
CGGCGAT12100.017.5506341
CGCCGTA377300.017.05028360-64
CCGATCT203450.017.03138715-19
TCGCCGT384100.016.69558360-64
GCCGTAT384800.016.63130260-64
CGTATCA391500.016.32480865-69
Produced by FastQC (version 0.11.5)