FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005403578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174118662
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG494950.0126.517451
GCGCAAC791600.0122.189961
TCGGTCG347700.0121.809082
CGGACGT528700.0121.607091
TTGCGCG272400.0121.305522
TACGGGC1155100.0120.420991
ACGCCGA643400.0119.6500241
TTACGCG425200.0118.981999
CTTACCG702850.0116.88211
AACGTCG240150.0116.6039665
CGCCGAC454500.0116.526352
TCCGCGA306500.0116.218668
CCTTACG701050.0115.409731
TTCGGTC842850.0115.098341
CGTTACG200500.0114.861387
CGCGGTA315900.0114.766471
GACGTTA782650.0113.911721
TTTGCGC665300.0112.909851
GTTACGC457150.0111.472518
GTCGCCG376550.0110.1940849
Produced by FastQC (version 0.11.5)