FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27613843
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG57550.0135.533089
AACGTCG67700.0131.286334
CGCGTTT140700.0129.987551
CTCGTAC164000.0128.72471
CGTACGT84750.0128.601043
CGAATCG79800.0128.186374
GCGGTCG103950.0127.891195
AACCGCG75050.0127.885891
GCGACGT63350.0126.5572059
CGCTCGA91000.0126.495455
CGAAGCG102700.0125.851759
CGGGTCG89650.0125.700042
GTTACGC101350.0125.3072368
AGCGCGA96900.0124.927638
CGTCCGA83200.0124.414416
CCGCTCG94150.0123.1248864
GTCCGCA172300.0123.0570148
CGCGGTC89800.0122.549944
ATCGACG42800.0121.434257
CGAGTCG54300.0121.347225
Produced by FastQC (version 0.11.5)