FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005403583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170618218
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC456550.0114.4026645
CGACGCT461100.0112.504216
CACGACG474050.0110.5112464
GACGCTC581100.089.720647
ACACGAC595400.088.356043
CTACACG609000.087.226841
TACACGA617100.085.5260242
ACGCTCT666900.078.1888058
CGCTCTT802250.064.922819
TTCGCGA59400.027.8349342
TCGCGAC64900.025.470533
CGCGAAA79600.025.2945331
TTTCGCG73100.022.2159081
CGCCGTA3770750.020.05269460-64
TCGCCGT3792650.019.84439560-64
GCCGTAT3818250.019.71172360-64
CGTATCA3847600.019.5259465-69
GTCGCCG3890400.019.37000860-64
TGGTCGC4001750.018.8984455-59
CCGTATC3845550.018.70613965-69
Produced by FastQC (version 0.11.5)