FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27665205
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA79850.0129.68142
CGTACCG55700.0128.189777
CCGATCG58750.0127.967524
TAACGCG50650.0126.935442
TAGTCGA166750.0126.06795
TATCGCG60200.0125.711896
TCGCGAG120500.0125.323061
CGGCTAG226650.0125.098411
ACGTACG72250.0124.811586
CTTACCG157000.0124.354641
CGTACGT72950.0124.110787
TAGGTCG127050.0124.04149
TCGTCGC50800.0119.2929846
CGCAACT128150.0118.9132
TCTACGG145650.0117.853798
GATCGAT142200.0117.815591
ATCGTTA180400.0117.573697
CTAGTCG81700.0116.319625
CGCTATC129950.0116.2657851
GTACCGT133400.0115.526018
Produced by FastQC (version 0.11.5)