FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27665205
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC120100.0125.547335
CACGACG122550.0123.580964
CGACGCT122000.0122.93966
GACGCTC140200.0107.187077
ACACGAC150000.0101.500043
CTACACG150850.0101.262791
TACACGA155650.098.166552
ACGCTCT161450.093.1684348
CGCTCTT180450.083.155549
TCCGATC154200.019.11436815-19
TTCCGAT161400.018.75570710-14
CTTCCGA166350.018.51135410-14
CGACGGT13000.017.5210061
CGGCGAT11700.016.9558141
TCTTCCG182650.016.80380610-14
CGCCGTA368700.016.6766260-64
CCGATCT180700.016.4877215-19
TCGCCGT371100.016.45547160-64
GCCGTAT377050.016.35729460-64
CGTATCA380600.016.07981965-69
Produced by FastQC (version 0.11.5)