FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32269584
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA423680.13129391441798569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2338550.0105.313251
AGCAGTG2594150.094.8042452
CAGTGGT3289100.074.665874
GCAGTGG3473600.070.944523
AGTGGTA3462950.070.926035
GTGGTAT3482500.070.5433356
GGTATCA3535350.069.629398
TGGTATC3524650.069.5144357
GTATCAA3648500.067.556569
CAACGCA3166750.039.0559112-13
GCAGAGT3174750.038.71836516-17
ATCAACG3524200.038.6535910-11
AGAGTAC3522600.038.49478518-19
TACATGG3481450.037.9967722-23
AGTACAT3573650.037.48975420-21
CGAAGAG156350.037.3790316-17
ACGCAGA3363750.036.81837514-15
GAGTACA3397600.035.812220-21
CGCAGAG3122350.035.78149816-17
TCAACGC3212300.034.93020612-13
Produced by FastQC (version 0.11.5)