FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28869791
Sequences flagged as poor quality0
Sequence length126
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA373050.0115.4271851
GTAACTC304200.0113.720453
CGCATTC330450.0113.4379046
CGTCTAC214500.0112.7763751
ACATACG344400.0112.6135941
TTGAACG361950.0112.413041
TGACTAG280000.0112.240261
CTGTACG228400.0111.749349
CGAAGTG271650.0111.442274
AGTAACT311250.0111.1446152
GATCTAG217300.0110.963911
AGCGACT191450.0110.923532
CAGTAAC323400.0110.715371
AGTGTCA221850.0110.6101151
GCGACTC192550.0110.445663
AGTATCG232000.0110.429088
CGCGATA188800.0110.382772
ACTCCGA181950.0110.348476
GGCGTCA192400.0110.313453
TAGTCTG231200.0110.25425
Produced by FastQC (version 0.11.5)