FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33265933
Sequences flagged as poor quality0
Sequence length126
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCG386700.0114.009431
TCAACGA470650.0113.069231
GAAGATC209300.0109.2083746
GCGACAC172650.0108.687794
ACTGATG166900.0108.59821
GACGTGC213350.0108.4309841
GGCGATA235900.0108.145489
GCGACCA255200.0108.040441
CCTTCGA263350.0107.957961
GTCGTAA247300.0107.1933751
CTGAAAC374400.0107.10391
GACCATC167200.0107.0263753
CCGTGGA188400.0106.883931
GACCTTA149300.0106.875462
CATCGAA193500.0106.736011
AGCGACA182450.0106.4349063
AGCAGCC382350.0106.259171
CTTCGAC159250.0106.038632
CTCGAAA221550.0105.881531
CAACGAG222750.0105.794392
Produced by FastQC (version 0.11.5)