FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36782046
Sequences flagged as poor quality0
Sequence length126
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG148150.0104.8637161
TACGGTA184700.0101.305381
TGCTACC122000.0100.475111
CGCTATC139400.0100.163511
GGATTAC186100.0100.15621
CCGTACT143450.099.930111
CGTGAGC103600.098.9091341
ACTGAAC135200.098.568671
GAACCTA142150.098.183541
TGACGGC114750.097.773061
ATGCGAT123900.097.6746441
CTGCCTA108400.096.8552551
TGACAAC180650.096.631581
TCTAACT223700.096.340958
CTGATAG97050.096.244671
TCGGTAA155300.095.629271
GCCTCTA128450.095.570051
CTGATCC112100.095.479111
GGCTCGA111900.095.381541
ACTGCTC109150.095.089781
Produced by FastQC (version 0.11.5)