FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36782046
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1349150.083.578721
AGCAGTG1495100.075.3213042
CAGTGGT1853750.060.6781164
GTGGTAT1850350.060.634536
GGTATCA1910800.058.7482228
TGGTATC1952150.057.3903277
GTATCAA1957150.057.3120469
AGTGGTA2033450.054.95015
GCAGTGG2153950.052.629843
CAACGCA1802850.035.04472712-13
ATCAACG1841150.034.41049610-11
AGAGTAC1899700.032.29910318-19
TACATGG1873450.031.98151222-23
GCAGAGT1956600.031.44852616-17
AGTACAT1943250.031.25218620-21
TCAACGC1804400.031.05873312-13
CGCAGAG1866050.029.74557916-17
GAGTACA1843050.029.33050520-21
TATCAAC1941100.028.86415310-11
GTACATG1869000.028.6476122-23
Produced by FastQC (version 0.11.5)