FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41272010
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1252700.076.281921
AGCAGTG1341100.071.037892
GGTATCA1467100.064.385818
GTGGTAT1482300.063.904416
TGGTATC1519400.062.207527
CAGTGGT1540600.061.7170944
GTATCAA1550300.061.02819
AGTGGTA1808250.052.3265535
GCAGTGG1980050.048.531793
CAACGCA1398650.037.60724612-13
ATCAACG1402550.037.52721810-11
TCAACGC1384050.034.27896512-13
TACATGG1513300.033.33244722-23
CGCAGAG1448200.032.6293416-17
AGAGTAC1580150.032.5735818-19
GCAGAGT1592350.032.5178116-17
AGTACAT1583850.032.23554220-21
GAGTACA1476750.031.22227320-21
GTACATG1500300.030.59059722-23
TATCAAC1576650.030.05449110-11
Produced by FastQC (version 0.11.5)