FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35405854
Sequences flagged as poor quality0
Sequence length126
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA241250.0111.3414466
CGTCGAC209000.0109.862297
GTCGACT218550.0109.17978
CCGAATC227250.0109.096013
ATCCGAA240150.0107.858481
AATGATC198950.0107.816333
TACTAGC188750.0107.634434
GCATACG205550.0107.15672
CCCGATG213600.0107.1351242
TCCCGAT297600.0107.0262761
CTGATCC208100.0106.672121
CGTCTAC183300.0106.564161
CAGCGAC224650.0106.482451
ATCTTAC234650.0106.3164447
CTAGTCG180250.0106.219144
GTGTCGC257450.0106.059868
GAGCATC164750.0105.906212
CGTGTCA201050.0105.7351763
CGGAGTG176350.0105.710224
CATACGT297250.0105.709293
Produced by FastQC (version 0.11.5)