FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35405854
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA646680.18264776214690373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1672850.091.9810641
AGCAGTG1835600.083.886522
CAGTGGT2200250.069.9106754
GTGGTAT2269450.067.784316
GGTATCA2275600.067.461018
TGGTATC2291600.066.9427
GTATCAA2341900.065.560439
AGTGGTA2433200.062.9192965
GCAGTGG2532850.061.157863
CAACGCA2208750.038.71144512-13
ATCAACG2219000.038.54407510-11
TACATGG2286450.035.9587322-23
AGAGTAC2339650.035.81464418-19
GCAGAGT2363450.035.61990716-17
AGTACAT2342100.035.40882520-21
TCAACGC2199450.035.05838412-13
CGCAGAG2255950.033.9403716-17
GAGTACA2264350.033.10536220-21
ACGCAGA2590350.033.0464514-15
GTACATG2278850.032.74230222-23
Produced by FastQC (version 0.11.5)