FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34977202
Sequences flagged as poor quality0
Sequence length126
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCTA158050.0105.926131
CTCTACG167800.0104.994191
CGCTATC172000.0104.6988451
ACTGATG130400.0101.871551
GACTACA163650.0101.0542761
GACGTGC138950.0100.959981
GACTGTA145450.0100.897719
CTGATAG110350.0100.4172741
AGCGTAT153850.0100.0000461
CTTACCG180850.099.708121
GCTATCC110200.099.63742
AGCTGCG124750.099.3642658
TACTCGC120100.099.2625351
ACGCCGA167450.099.191191
GATCTAG138250.099.1699451
GCAGTCT147150.099.0423054
TCTTGAG167600.099.019018
CCTTCGA145700.098.466261
GACCAAT196350.098.165411
CTAATAG120950.097.726843
Produced by FastQC (version 0.11.5)