FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34977202
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG362990.10377902726467372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1162650.085.370771
AGCAGTG1311950.075.534972
CAGTGGT1638450.060.4282464
GTGGTAT1645750.060.037626
GGTATCA1687900.058.5398
TGGTATC1725550.057.126297
GTATCAA1758850.056.232819
GCAGTGG1894900.052.7262233
AGTGGTA2043650.048.2415285
CAACGCA1591500.034.5690112-13
ATCAACG1612700.034.18008810-11
AGAGTAC1706050.031.24725718-19
TCAACGC1585750.031.15381212-13
TACATGG1665900.031.14964922-23
AGTACAT1696650.031.0128620-21
GCAGAGT1738800.030.8223716-17
CGCAGAG1652250.029.68863516-17
GAGTACA1634400.029.0781520-21
TATCAAC1743550.028.3502410-11
GTACATG1664450.028.30452722-23
Produced by FastQC (version 0.11.5)