FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40515114
Sequences flagged as poor quality0
Sequence length126
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC215250.0107.8755651
AGCTACC186000.0106.963288
ATCGTTG183150.0104.631187
GATGATC178400.0104.425833
ACTGAGT267200.0104.044241
CAGCTGT257200.0103.316068
TCGAATG176000.0103.154798
AGCTGTG153100.0103.065469
TCGTTAC158800.0102.161988
CCTGTAG264450.0102.139699
CAAGTTG298450.0102.121061
AGACTAG149350.0101.917418
AAGACTA192300.0101.867827
CGGACGT210800.0101.666381
GCAGTTA153400.0101.59191
ACTGATG133100.0101.571081
TCCTGTA321800.0101.3692868
CCGTGGA192250.0101.171551
CACGCGA144400.0101.1315168
AGTGTAC219100.0100.990768
Produced by FastQC (version 0.11.5)