FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40515114
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT982400.069.652181
GGTATCA1059200.064.714198
GTGGTAT1087550.062.9610446
GTATCAA1139750.060.2037779
TGGTATC1139950.060.047737
AGCAGTG1165650.058.816672
CAGTGGT1208900.056.7471964
AGTGGTA1434900.047.5252725
GCAGTGG1557200.044.5611083
CAACGCA963600.039.0940412-13
ATCAACG980750.038.5690110-11
TCAACGC955750.035.84078612-13
CGCAGAG1044600.032.60863516-17
AGAGTAC1138100.032.3056418-19
TACATGG1127950.032.07976522-23
GAGTACA1053150.031.59474220-21
GCAGAGT1188450.031.14248716-17
AGTACAT1184250.030.88579820-21
TATCAAC1136500.030.33294910-11
GTACATG1118550.029.58988422-23
Produced by FastQC (version 0.11.5)