FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40379468
Sequences flagged as poor quality0
Sequence length126
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTA335350.0109.681631
GCCTCTA341150.0108.098441
AGATTGC230300.0106.483531
GTGACGC185550.0106.383298
GTGCATA209250.0106.350971
TGCATAC196700.0105.600242
TATGCCC282000.0104.1409761
CGCTGGA224150.0103.5666051
CTGGATC169000.0103.382853
GACACGC279950.0103.273841
TGACGCC192050.0102.687979
CGTTAGA197550.0100.555091
AAAGTAG325400.099.918391
ATGTATC162100.099.899593
GCAGTTA183950.099.700131
ATTCACG174450.099.5665366
TCACGCG151250.099.448328
TGACTAG165350.099.406221
TCTGGAA283250.099.188261
ATAACGC164800.099.155141
Produced by FastQC (version 0.11.5)