FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40379468
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1086750.071.624241
GGTATCA1228800.063.065578
AGCAGTG1247950.062.3422662
GTGGTAT1253050.061.873376
TGGTATC1311300.059.0766037
GTATCAA1326150.058.499429
CAGTGGT1367900.056.966464
AGTGGTA1463850.052.8104935
GCAGTGG1710500.045.8940353
CAACGCA1135500.037.63448712-13
ATCAACG1162950.036.83826410-11
TCAACGC1127700.034.39734612-13
TACATGG1266400.031.85511822-23
CGCAGAG1219950.031.70066316-17
AGAGTAC1321400.031.40870518-19
GCAGAGT1339350.031.15207716-17
GAGTACA1208750.031.00670420-21
AGTACAT1352650.030.4378820-21
TATCAAC1318500.029.60790810-11
GTACATG1268400.029.2508522-23
Produced by FastQC (version 0.11.5)