FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39034463
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1104550.076.5508351
AGCAGTG1244700.067.984622
GTGGTAT1362600.061.975686
GGTATCA1369850.061.7356878
CAGTGGT1437700.058.8865054
TGGTATC1441300.058.588637
GTATCAA1455600.058.173719
AGTGGTA1625550.051.7791635
GCAGTGG1740700.049.105683
CAACGCA1288550.036.67272612-13
ATCAACG1335800.035.52363610-11
TCAACGC1293750.032.79049312-13
TACATGG1380450.032.1220522-23
AGAGTAC1434150.031.89220218-19
GCAGAGT1451450.031.68454716-17
AGTACAT1458350.031.03102520-21
CGCAGAG1379300.030.58464416-17
GAGTACA1337800.030.45282220-21
GTACATG1367750.029.33903722-23
TATCAAC1467400.028.95704810-11
Produced by FastQC (version 0.11.5)