FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38139668
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1156400.079.076741
AGCAGTG1285450.071.060172
GTGGTAT1404600.064.736466
GGTATCA1415550.064.241678
CAGTGGT1474700.061.824484
TGGTATC1486750.061.104997
GTATCAA1515550.060.185439
AGTGGTA1651650.054.874145
GCAGTGG1759750.052.328813
CAACGCA1338650.037.67488512-13
ATCAACG1382900.036.64005310-11
TCAACGC1338250.034.02174412-13
TACATGG1430150.033.1091622-23
GCAGAGT1509700.032.55581716-17
AGAGTAC1511800.032.33312218-19
AGTACAT1502200.032.19351220-21
CGCAGAG1426950.031.75963416-17
GAGTACA1386700.031.67146520-21
GTACATG1418950.030.49766522-23
TATCAAC1534500.029.78543710-11
Produced by FastQC (version 0.11.5)