FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35867981
Sequences flagged as poor quality0
Sequence length126
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAG207600.0105.422991
GTGCAGC174550.0102.343221
TGGACGC163500.0102.174251
CGAGCAC140150.0101.208411
GGCGACT169900.0100.515931
CGTTAGA156800.0100.4914861
CAACTAG180850.0100.172091
GACTAAC118100.099.672121
GATGCTA96750.099.3933941
GTAACTG131450.099.276151
TTAGTTC169500.099.0178451
TCAGGAG162000.098.294193
CTTAGGA164650.098.2888261
CTAAGAC125100.098.173511
GAAGTCG97400.098.067844
CGTGAGC112800.097.9162451
TGACGGC92150.097.906081
ATAGACC115150.097.690351
GCGCGAT130450.097.092211
GATCTAG133950.096.8855441
Produced by FastQC (version 0.11.5)