FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38516468
Sequences flagged as poor quality0
Sequence length126
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG167800.0112.317541
TAACGTA289150.0110.955762
CGGCCGA379650.0110.498279
CCGTTCA328400.0109.514581
CGTTGAG216600.0109.4766163
TGGACGC220850.0108.636641
GAGTCCG359950.0108.6226651
AGTGTAC163500.0108.473638
CACGCGA255150.0108.309338
CACATAG241900.0107.895171
TCGGCCG390100.0107.6459058
TTACGCG195950.0107.381149
TCCCGAT341300.0107.380771
AACGTTG305050.0107.209831
CATGCTA201600.0107.079938
ACGTTGA221500.0106.946432
GTCTTAG224450.0106.873977
GTTACGC197200.0106.791748
CCCGATA225850.0106.746272
CGTGTCA183200.0106.607663
Produced by FastQC (version 0.11.5)