FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36510494
Sequences flagged as poor quality0
Sequence length126
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATAC351150.0113.597881
GATGCTA280950.0112.769411
ACGGAGA396700.0111.996071
GTCGTAA278000.0110.510581
CGGAGAC294500.0110.4064942
CGAGCAC274350.0109.96751
AGCTACC224100.0109.286758
AGATCGA222450.0109.180348
AGCACGA217300.0108.4294056
CTGCCTA215500.0108.153741
GATGTGA268500.0108.002375
TGAGCGA145400.0107.410499
GCACGAA220100.0107.2411357
CAATGGA302650.0107.2290958
CTGCGGA162500.0107.035641
GCAGTTA193750.0106.877131
GTCGAGT242000.0106.632718
GCCTCTA219650.0106.4383241
GTGAGCG146800.0106.426878
AGTCCTG201200.0106.314462
Produced by FastQC (version 0.11.5)