FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36519532
Sequences flagged as poor quality0
Sequence length126
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC251650.0110.607241
ACGCCGA287950.0110.216631
GCCTCTA227900.0107.222791
GACTAAC179950.0106.8999941
CTGAAAC282050.0106.7321551
CTACGTC227850.0106.455821
TCACATC248500.0106.313275
GATGATC151400.0105.5780263
GCGTATC120350.0105.445852
AGATCAC321350.0105.32785
GATCACC231350.0105.241336
AGAGATC222600.0105.151633
ACTGATG128750.0104.876761
AGTATTG210800.0104.8532948
AGCGTCG105200.0104.441331
CCAGCGA188550.0104.2213061
GAGATCA223700.0104.124944
AGCTTGA133850.0103.8411941
GTAACGT310350.0103.267551
GTATATG187450.0102.9671558
Produced by FastQC (version 0.11.5)