FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36519532
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1153800.078.946381
AGCAGTG1248150.072.9174352
GGTATCA1441700.062.868788
GTGGTAT1461650.062.1112486
TGGTATC1483250.061.132117
CAGTGGT1493400.061.0157434
GTATCAA1517200.059.8395929
AGTGGTA1709800.052.9494445
GCAGTGG1784350.051.3855633
CAACGCA1367650.036.86305212-13
ATCAACG1394300.036.2583810-11
TCAACGC1371350.033.21824312-13
AGAGTAC1509350.032.7015218-19
TACATGG1484000.032.47883622-23
AGTACAT1518550.032.29678320-21
GCAGAGT1555050.031.9335216-17
CGCAGAG1442950.031.44811616-17
GAGTACA1440250.030.77043520-21
GTACATG1465650.029.88732522-23
TATCAAC1546800.029.43389510-11
Produced by FastQC (version 0.11.5)