FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37724523
Sequences flagged as poor quality0
Sequence length126
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA457500.0112.715141
CCTAGCA385300.0110.908343
CAGACGC391050.0110.820818
CACGTGA288950.0110.0082248
ACTGATG225600.0109.884531
GTGATGC233900.0109.8635948
GTCGATG201900.0109.805597
TAGAGGC348200.0109.47415
AGCTCTC522550.0109.460921
GAATGTC266600.0109.3348543
CGCTATC218000.0109.308861
CTGATAG228750.0109.263741
GTACAGC325900.0108.9561845
GCATGCG285000.0108.8911061
CTCGTCA366650.0108.844061
TCACGTG289750.0108.81677
GTGCATC270300.0108.6088268
GTGCAGC248050.0108.483421
TCGTACA307600.0108.474543
TCGTCGT212350.0108.2985158
Produced by FastQC (version 0.11.5)