FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37724523
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1242600.079.4857941
AGCAGTG1347900.073.0759662
GGTATCA1501750.065.492528
GTGGTAT1522650.064.331856
TGGTATC1533800.063.807917
CAGTGGT1570700.062.605514
GTATCAA1587600.062.087349
AGTGGTA1823500.053.73545
GCAGTGG1957700.050.6634753
CAACGCA1428050.038.36273212-13
ATCAACG1447750.037.8910310-11
TCAACGC1427000.034.58233612-13
TACATGG1551250.033.6971422-23
AGAGTAC1585750.033.67103218-19
AGTACAT1588850.033.34892720-21
GCAGAGT1620400.033.2529316-17
CGCAGAG1499450.032.74656716-17
GAGTACA1511450.031.71871620-21
GTACATG1532950.030.98434422-23
TATCAAC1624900.030.42282510-11
Produced by FastQC (version 0.11.5)