FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33051544
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1264400.085.2813641
AGCAGTG1378050.078.144572
GTGGTAT1688550.063.6735956
CAGTGGT1709450.062.956164
GGTATCA1709800.062.9018758
TGGTATC1758300.061.057647
GTATCAA1786250.060.3175549
AGTGGTA1808750.059.2112925
GCAGTGG1904600.056.9291273
CAACGCA1646700.036.60600712-13
ATCAACG1677350.036.04667310-11
TACATGG1716700.033.45824422-23
AGAGTAC1770000.033.21280318-19
GCAGAGT1794350.032.98114416-17
AGTACAT1767900.032.89156320-21
TCAACGC1652000.032.7429412-13
CGCAGAG1727200.031.17568816-17
GAGTACA1686550.031.06868620-21
ACGCAGA1987650.030.40378814-15
GTACATG1713450.030.33916522-23
Produced by FastQC (version 0.11.5)