FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36848886
Sequences flagged as poor quality0
Sequence length126
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGCG363650.0112.2252961
TCGAGGC281500.0111.536361
GTACGTA303800.0111.352351
TCGAATG245800.0109.06088
AGCGTGA216100.0109.057138
AATGTAG331400.0108.8326643
GCAGTTA194650.0108.562911
TATGCCC325750.0108.235261
AAGTGTC266700.0108.232576
ATGGCGA180250.0108.0533752
ACGATAC233200.0107.993251
GTGCAGC218200.0107.961151
TAGGCTA200050.0107.82157
TTCGCGA198950.0107.299919
TCTATTG279600.0107.141231
ATAGGTC214950.0106.967814
CGCACTC199150.0106.921019
AGAGTTC242650.0106.62613
AGTGTCG162050.0106.5581747
GCCTCTA208850.0106.47061
Produced by FastQC (version 0.11.5)