FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36848886
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1239800.081.4696351
AGCAGTG1341900.075.1867142
GGTATCA1535300.065.462898
GTGGTAT1555300.064.654756
CAGTGGT1576750.064.0573964
TGGTATC1572800.063.8354157
GTATCAA1618350.062.2413989
AGTGGTA1813500.055.3837935
GCAGTGG2014400.050.6009753
CAACGCA1457800.038.0854212-13
ATCAACG1470000.037.88644810-11
TCAACGC1451400.034.68015312-13
AGAGTAC1594200.033.97294218-19
TACATGG1576400.033.64740822-23
AGTACAT1605150.033.47295420-21
GCAGAGT1643750.033.1552516-17
CGCAGAG1525250.032.85598416-17
GAGTACA1531700.031.91730120-21
TATCAAC1618600.031.16527410-11
GTACATG1558750.031.1264722-23
Produced by FastQC (version 0.11.5)