FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37533521
Sequences flagged as poor quality0
Sequence length126
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCAG423200.0111.864353
ATAACGC333500.0111.746941
ACGAGCC349650.0111.462251
CTGCGGA452250.0111.160951
ATCGATC214300.0110.986942
ATGCCTC272250.0110.812242
CGGACTG256500.0110.27461
CGAGCAC310200.0109.8240051
AGTGTAC205600.0109.753698
GTCGTTG343950.0109.39078
CTTACCG287800.0109.338511
TACTTAC274150.0109.285371
ACGTGAG259850.0109.167599
TCGATCA217800.0109.093193
AGTCGGA258250.0108.405537
ACTAGTG170600.0108.0796365
TCACGTG202950.0107.818617
AGTATCT327750.0107.6033559
ACGTAGA204900.0107.586444
GATCATC220500.0107.349195
Produced by FastQC (version 0.11.5)