FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37533521
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1076250.074.9897461
AGCAGTG1168200.068.858352
GGTATCA1282350.062.7111138
GTGGTAT1299150.061.607146
TGGTATC1321400.060.40547
GTATCAA1352800.059.5830769
CAGTGGT1349400.059.5603074
AGTGGTA1621900.049.2833755
GCAGTGG1705800.047.646223
CAACGCA1207450.036.90065812-13
ATCAACG1216800.036.73393210-11
TCAACGC1196900.033.71507312-13
AGAGTAC1349150.032.0362418-19
TACATGG1320000.032.0127822-23
CGCAGAG1277050.031.49185616-17
AGTACAT1362650.031.41364320-21
GCAGAGT1417300.030.81778116-17
GAGTACA1269500.030.68251220-21
GTACATG1311450.029.4242922-23
TATCAAC1376900.029.37824610-11
Produced by FastQC (version 0.11.5)