FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33610094
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1216500.086.135461
AGCAGTG1380650.075.8390662
GTGGTAT1656750.063.0925566
CAGTGGT1679500.062.6223874
GGTATCA1702650.061.366088
AGTGGTA1731100.060.4195985
GTATCAA1763450.059.2712489
TGGTATC1788350.058.5400357
GCAGTGG1891350.055.9904563
CAACGCA1601800.036.39386712-13
ATCAACG1665350.035.0442610-11
AGAGTAC1710600.033.07475718-19
TACATGG1687000.032.9689722-23
AGTACAT1726150.032.57451220-21
GCAGAGT1757150.032.3739716-17
TCAACGC1612200.032.37294812-13
ACGCAGA1865450.031.13088614-15
CGCAGAG1681550.030.77902616-17
GAGTACA1660550.030.52129620-21
GTACATG1683500.029.97287622-23
Produced by FastQC (version 0.11.5)