FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38337773
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1747800.093.77211
AGCAGTG1916850.085.520352
GGTATCA2213900.073.577718
GTGGTAT2222100.073.486496
CAGTGGT2269300.072.405574
TGGTATC2259700.072.138787
GTATCAA2295350.071.040279
GCAGTGG2554000.064.707243
AGTGGTA2548550.064.2130055
CAACGCA2141250.041.9516312-13
ATCAACG2156600.041.5698510-11
AGAGTAC2248900.039.0081318-19
TACATGG2239450.038.51968422-23
AGTACAT2271100.038.30935720-21
TCAACGC2131700.038.14202512-13
GCAGAGT2316300.037.99671616-17
CGCAGAG2194200.036.83561316-17
GAGTACA2215550.035.89325320-21
GTACATG2219500.035.69939822-23
TATCAAC2316100.035.19263510-11
Produced by FastQC (version 0.11.5)