FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39873911
Sequences flagged as poor quality0
Sequence length126
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATC430750.0113.092269
CCTTACG317300.0111.534541
CGGACGT345450.0111.339671
GGTGATC321250.0109.832373
TCGGAAT448150.0108.714568
GCGTATC199800.0108.611382
CTGATAG213350.0108.2178651
GTCTCAG370250.0108.162955
AGGGTGA457300.0107.734791
GATGCGA183150.0107.473519
CGCTATC167800.0107.151
CTGATCC229150.0106.8914641
AGCATGA257450.0106.890239
ACGGGTC381650.0106.876181
AGCGTAT268200.0106.8732451
TCAGTGC343800.0106.828988
GTCGACT202750.0106.7892768
CAGTGCA344750.0106.430329
CGCATTC240900.0106.0923846
AGTCGTA285850.0106.0738756
Produced by FastQC (version 0.11.5)