FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29828000
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1009950.077.9837651
AGCAGTG1010350.077.4450152
GTGGTAT1082650.072.1897056
GGTATCA1081550.072.129638
TGGTATC1122850.069.338037
GTATCAA1131000.069.273019
CAGTGGT1198600.065.371514
GCAGTGG1397350.056.387023
AGTGGTA1428800.054.7090645
CAACGCA1012750.042.1170512-13
ATCAACG1024850.041.6607710-11
TCAACGC1005000.038.9137212-13
AGAGTAC1129750.037.35647218-19
TACATGG1107300.037.26620522-23
CGCAGAG1066700.036.6622416-17
AGTACAT1155300.036.4164320-21
GCAGAGT1174200.036.2055716-17
GAGTACA1076150.035.8778120-21
GTACATG1110300.034.56892422-23
TATCAAC1159300.033.85342410-11
Produced by FastQC (version 0.11.5)