FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32175828
Sequences flagged as poor quality0
Sequence length126
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGA257400.0113.348541
CCGTGGA246450.0112.8575061
GCGAGTC191950.0110.9918443
ACGAGCC216800.0110.9650651
AGGTCCG199950.0110.682511
CTAGAGA192050.0110.465454
TCCACGA247300.0110.410558
ACTAGAG194150.0109.7341463
TCACGCG156350.0109.632728
ACGAGGA143500.0109.561741
TCGCGAG213300.0109.325491
GCTCGAC151650.0109.0062942
CTCGACA152050.0108.826933
GTAACTG244400.0108.795291
AACTAGA192700.0108.609332
GCTCGAG111300.0108.5228042
CGCGAGT195650.0108.5051652
TCACGAA241950.0108.3592451
GTGACGG116300.0108.283078
CGAGTCC192500.0108.274854
Produced by FastQC (version 0.11.5)