FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32175828
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG437640.13601514776869145No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC361270.1122799388410455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1376550.093.20981
AGCAGTG1486150.085.936822
GGTATCA1652550.077.192118
GTGGTAT1660900.076.897966
TGGTATC1671650.076.037367
GTATCAA1687800.075.871429
CAGTGGT1733550.073.626844
GCAGTGG1962800.065.3514253
AGTGGTA2047350.062.344995
ATCAACG1593150.044.29615410-11
CAACGCA1592900.044.1730612-13
AGAGTAC1716850.040.7080818-19
TACATGG1683500.040.7042822-23
TCAACGC1579900.040.44857412-13
AGTACAT1745800.039.87362720-21
CGCAGAG1637200.039.0293416-17
GAGTACA1658100.038.1831220-21
GCAGAGT1928300.036.6642716-17
GTACATG1724450.036.5871322-23
TATCAAC1760450.036.44513310-11
Produced by FastQC (version 0.11.5)