FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29962201
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG332800.11107328196616797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1138400.084.690851
AGCAGTG1180500.081.2402042
GTGGTAT1282550.074.663826
GGTATCA1288700.074.261078
GTATCAA1322750.072.567189
TGGTATC1325000.072.077057
CAGTGGT1401150.068.5494464
AGTGGTA1592250.060.17155
GCAGTGG1603950.060.117853
CAACGCA1215900.043.76494612-13
ATCAACG1220650.043.68560410-11
TCAACGC1203200.039.84384512-13
TACATGG1306650.039.6230322-23
AGAGTAC1341550.039.3098418-19
AGTACAT1352700.038.83745220-21
GCAGAGT1379150.038.4616-17
CGCAGAG1273000.037.67022316-17
GAGTACA1278550.037.05172320-21
GTACATG1310300.036.06691722-23
TATCAAC1369650.035.11126710-11
Produced by FastQC (version 0.11.5)