FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32425432
Sequences flagged as poor quality0
Sequence length126
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG665350.0117.478221
AGTATCG1122850.0117.418168
CTGATGA654950.0117.090862
GTATCGA1126100.0117.084799
AGCCTAA879850.0116.991211
TGATGAG659400.0116.673133
GACGACG351750.0116.446019
AGACGAC352800.0116.4053658
CCGGTAG428200.0116.2568361
GATGAGA662600.0116.254534
ATAGACG351500.0116.204346
GAGAGTA664050.0116.189887
GAAACTC1252500.0116.0599751
GCCTAAA870850.0115.8838962
ATGAGAG662450.0115.846135
CTCAGTA1131600.0115.821515
ACTGAAG652450.0115.764163
GGGCATG875900.0115.760659
GCCTGTA595250.0115.589514
ACGATCG271900.0115.514794
Produced by FastQC (version 0.11.5)