FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32425432
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG545520.16823831367921327No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA494790.15259318673071187No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC425220.13113780565822533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1934050.0100.2278061
AGCAGTG2067800.093.349382
GGTATCA2283300.084.420218
GTGGTAT2299950.083.83796
TGGTATC2301650.083.405577
GTATCAA2327250.083.119849
CAGTGGT2386800.080.797174
GCAGTGG2515250.076.862163
AGTGGTA2583200.074.600885
ATCAACG2222800.047.42148210-11
CAACGCA2233300.047.12591612-13
AGAGTAC2331400.044.7711318-19
TACATGG2314050.044.0958922-23
AGTACAT2366050.043.9129120-21
TCAACGC2217900.043.56588412-13
CGCAGAG2274300.042.48288316-17
GAGTACA2287250.041.78874220-21
GTACATG2329450.040.80243722-23
ACGCAGA2603300.040.7287614-15
TATCAAC2384500.040.63028310-11
Produced by FastQC (version 0.11.5)