FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32695143
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG541210.16553223211166257No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC438230.13403519905081926No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA391640.11978537607252551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1625950.097.356261
AGCAGTG1758800.089.640742
GGTATCA2009900.078.456118
GTGGTAT2024550.077.8350456
TGGTATC2018550.077.83167
GTATCAA2047300.077.248539
CAGTGGT2078250.075.826864
GCAGTGG2338850.067.570463
AGTGGTA2387550.065.978595
ATCAACG1951600.044.72107310-11
CAACGCA1953050.044.59103412-13
AGAGTAC2062950.041.91421518-19
TACATGG2051950.041.4213922-23
AGTACAT2110450.040.8360320-21
TCAACGC1940250.040.72152712-13
CGCAGAG1999450.039.49147816-17
GAGTACA2028100.038.5713520-21
GCAGAGT2303100.037.94887516-17
TATCAAC2105500.037.6010710-11
GTACATG2085850.037.3942222-23
Produced by FastQC (version 0.11.5)