FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35174296
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG607400.17268291595658375No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC538480.1530890625358927No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA522390.1485146994839641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2135050.0101.19781
AGCAGTG2311150.093.1020052
GGTATCA2613700.082.172868
GTGGTAT2619100.082.063236
TGGTATC2627950.081.446477
GTATCAA2659000.081.075289
CAGTGGT2684500.080.097534
GCAGTGG2867550.075.128873
AGTGGTA2926550.073.44825
ATCAACG2549900.046.28020510-11
CAACGCA2559350.046.0236612-13
AGAGTAC2682750.043.48182718-19
TACATGG2640300.043.11519622-23
AGTACAT2717400.042.69354620-21
TCAACGC2539400.042.33334412-13
CGCAGAG2612600.041.13792816-17
GAGTACA2630900.040.3935920-21
ACGCAGA2975450.039.81838214-15
TATCAAC2730250.039.5159810-11
GTACATG2671600.039.46927622-23
Produced by FastQC (version 0.11.5)